Insertion junction: LMJ.RY0402.043747_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.043747
Chromosome:chromosome 3
Location:2562890
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g160350 ANK7 (1 of 1) PF07714//PF12796 - Protein tyrosine kinase (Pkinase_Tyr) // Ankyrin repeats (3 copies) (Ank_2); Predicted protein with ankyrin repeats CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCCTGCCACTGCCGGTGCTGCCACCGC
Internal bar code:CGCATTGCGCTTCGGTTAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:150
LEAP-Seq percent confirming:64.539
LEAP-Seq n confirming:455
LEAP-Seq n nonconfirming:250
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGATTGTGATTTGGACGAA
Suggested primer 2:AGAGGGCGTTATGATGATGG