| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043752 |
| Chromosome: | chromosome 10 |
| Location: | 5532036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459450 | SUB10 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCTTGCTTTCTCGGTCTTGTGTTGTTA |
| Internal bar code: | TGCGGGAATCGGCGCGGGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 84.7685 |
| LEAP-Seq n confirming: | 1703 |
| LEAP-Seq n nonconfirming: | 306 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCAAGGAAGTGGTGGTGG |
| Suggested primer 2: | CGGTAAAATGGTGGGTATCG |