| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.043755 |
| Chromosome: | chromosome 1 |
| Location: | 7419151 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053288 | (1 of 1) K01974 - RNA 3'-terminal phosphate cyclase (ATP) (RTCA, rtcA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCGCTCGCCCTTGGCACTGGCGCCTGG |
| Internal bar code: | TTTGGGGGGGAGATTATGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 89 |
| LEAP-Seq percent confirming: | 83.7104 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCATGTACGGTGCTCCC |
| Suggested primer 2: | CCACCGACACACAATCAGAC |