Insertion junction: LMJ.RY0402.043761_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.043761
Chromosome:chromosome_12
Location:1216678
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g489500 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GCAGCATGTCACCCAGGAGCTTCTTCTTCA
Internal bar code:GAGAGCGATAAAACGTAGCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:475
LEAP-Seq percent confirming:94.3396
LEAP-Seq n confirming:450
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTGACTCCACTCGTTCAG
Suggested primer 2:GTCTCCGGTAGCAACACCAT