| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043784 |
| Chromosome: | chromosome 10 |
| Location: | 6082043 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAACCCCCGATCGTCAGAACCGCAACCC |
| Internal bar code: | TTGGGCGACTATGAACCTCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 82.8402 |
| LEAP-Seq n confirming: | 560 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGAACCTGCCAAGGTAT |
| Suggested primer 2: | GTTGAGCAGGTGCTGGTACA |