| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043792 |
| Chromosome: | chromosome 2 |
| Location: | 7524993 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144006 | (1 of 17) PF13833 - EF-hand domain pair (EF-hand_8) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACTAGCACCCCCAGCACCCCCAGCAGC |
| Internal bar code: | TGGGTGGTTGACTGAACTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 82.5053 |
| LEAP-Seq n confirming: | 5862 |
| LEAP-Seq n nonconfirming: | 1243 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGTTCGGTTTCGTTTGG |
| Suggested primer 2: | CACCAATACTGGCACTGTGG |