Insertion junction: LMJ.RY0402.043798_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.043798
Chromosome:chromosome 6
Location:5766406
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g287300 COG9 ankyrin repeat protein; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACATGTCACGTGACGCCGCCGCCTGCAG
Internal bar code:GGCAAAGCACGCTGTCGCAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:38
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:120
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTTACGCTCTCTTGTTGCCC
Suggested primer 2:CAGGCTCGCTTCCTATTGAC