| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043815 |
| Chromosome: | chromosome 10 |
| Location: | 5983406 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462600 | PUS15 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCTCCTTCTTTGAATGTAACCGCCCAC |
| Internal bar code: | GGGGGCGTCACAACTGCCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 97.0246 |
| LEAP-Seq n confirming: | 2087 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTACTGCAAGGACTGGTG |
| Suggested primer 2: | CTCGAGCATAATCGGCTTTC |