Insertion junction: LMJ.RY0402.043817_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.043817
Chromosome:chromosome 7
Location:6284547
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g356900 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCCCAGTCGCCAGTTGCCCCCGCGCGCG
Internal bar code:TCGTTCCTTGCAAGCGCCCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:749
LEAP-Seq percent confirming:99.1202
LEAP-Seq n confirming:338
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGAATGAGATTGGACCGTGA
Suggested primer 2:AATACACGCATCCCAACACA