Insertion junction: LMJ.RY0402.043841_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.043841
Chromosome:chromosome 14
Location:3121422
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g628702 (1 of 1) PF11919 - Domain of unknown function (DUF3437) (DUF3437) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATCCTTCCCCAACCACCAACCACCACT
Internal bar code:ATGCCGAGCGGGTCGAACTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:278
LEAP-Seq percent confirming:97.3549
LEAP-Seq n confirming:3607
LEAP-Seq n nonconfirming:98
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGGTAGGATTGGGATTTG
Suggested primer 2:ATTCCTTCCCTAATGCCCTG