| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043864 |
| Chromosome: | chromosome 1 |
| Location: | 6255094 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044750 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCGGCATGGGCGATTGCTTTCAAGACT |
| Internal bar code: | TCCGGGAACTCTACGGGATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 99.3854 |
| LEAP-Seq n confirming: | 2264 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGGCAGTTCCAATATCA |
| Suggested primer 2: | ACTACAATGCTGCCATGCTG |