Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.043874 |
Chromosome: | chromosome 7 |
Location: | 4011210 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCTGCGCGGGGCCTGGGGGCGGACGGC |
Internal bar code: | AAGCCGAATGCCCCCTACGATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 335 |
LEAP-Seq percent confirming: | 79.7578 |
LEAP-Seq n confirming: | 7443 |
LEAP-Seq n nonconfirming: | 1889 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATGGCATATGCGAGTTTCC |
Suggested primer 2: | CTTACTTGGCTCCTTCGTGC |