| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043904 |
| Chromosome: | chromosome 3 |
| Location: | 5119957 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCACAGCGTGGCTGTGGGCGCTAGTGT |
| Internal bar code: | TCCCTCGGACCCAGGCTGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 99.3074 |
| LEAP-Seq n confirming: | 5592 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGCAAGAAGACGGTGCC |
| Suggested primer 2: | AACTTCAGCACCGTCGAGTT |