| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043937 |
| Chromosome: | chromosome 1 |
| Location: | 3972061 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025850 | INO | (1 of 1) 1.13.99.1 - Inositol oxygenase / Myo-inositol oxygenase; myo-inositol oxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGACGCTAAGTGCGAGGGCGGTGACGA |
| Internal bar code: | TGAATTTCTAAAAGTCGTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 776 |
| LEAP-Seq percent confirming: | 99.6025 |
| LEAP-Seq n confirming: | 9772 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATCAAATGACCACCTCG |
| Suggested primer 2: | GCTAGGTGCGAAGCATAAGG |