Insertion junction: LMJ.RY0402.043941_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.043941
Chromosome:chromosome 10
Location:1714258
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g430450 (1 of 1) 3.5.1.10 - Formyltetrahydrofolate deformylase / Formyltetrahydrofolate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGTCGGGACATGTGTCATACTTCCAATG
Internal bar code:AGGCGCGGAGGGAGCGCATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:469
LEAP-Seq percent confirming:98.8563
LEAP-Seq n confirming:5186
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGGATCGGGACTGAGATG
Suggested primer 2:GGCATGTCCACTGATACACG