Insertion junction: LMJ.RY0402.043942_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.043942
Chromosome:chromosome_10
Location:5829651
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre10.g461575 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CAGTACGCAGCACCTAGCGGCCTCACCGTC
Internal bar code:TGGGTCGATTTTGCAGGTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:574
LEAP-Seq percent confirming:99.3151
LEAP-Seq n confirming:1450
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGTAAAAGAGAGCACGGCAG
Suggested primer 2:AGGATTCGTGGTGCAAGAAC