| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.043946 |
| Chromosome: | chromosome 16 |
| Location: | 6792451 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674403 | (1 of 30) PTHR24349:SF79 - NITROGEN NETWORK KINASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCATCACCTTATTGCAACGAACCACTG |
| Internal bar code: | TTGCACTGTTTGGCAACAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 498 |
| LEAP-Seq percent confirming: | 99.6314 |
| LEAP-Seq n confirming: | 9730 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCCGCAACTAACTTTGC |
| Suggested primer 2: | GGCACTTACGACTCACCCAT |