| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043952 |
| Chromosome: | chromosome 14 |
| Location: | 461529 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610950 | PBD1 | 20S proteasome beta subunit D, type 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTACGACCACCACCATCGATCTGCCTC |
| Internal bar code: | AGTCGAGGGAGGCCGGCCAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 99.6239 |
| LEAP-Seq n confirming: | 5828 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCTGGGCCATGAGTAGT |
| Suggested primer 2: | CAAGCTAAGCCAAACCAAGC |