| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043954 |
| Chromosome: | chromosome 2 |
| Location: | 540232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077350 | HDH1 | Histidinol dehydrogenase; (1 of 1) 1.1.1.23 - Histidinol dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCTTGCTTGCTGGCTGGCGTGGACTCG |
| Internal bar code: | GCGTGGCACTCTCTCCTTCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 99.7116 |
| LEAP-Seq n confirming: | 5878 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTAGGTTGCGGAAGAACT |
| Suggested primer 2: | AAAATGCACTTACGTTGCCC |