| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043964 |
| Chromosome: | chromosome 10 |
| Location: | 603663 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421850 | (1 of 1) PTHR13542:SF0 - PROTEIN LSM12 HOMOLOG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCATCCTTCGTTCATACACATTCACCAC |
| Internal bar code: | CCGATAACCTTTAACCAAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 93.9394 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCAGCTGTTGAGGTTGA |
| Suggested primer 2: | TCTTTGGGACTGAGGATTGG |