| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043966 |
| Chromosome: | chromosome 12 |
| Location: | 8422674 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548550 | (1 of 1) PF05742 - Transport and Golgi organisation 2 (TANGO2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGATGGCAGCGGCATCATCGGCGGCCG |
| Internal bar code: | CGCGATTAGTTTAGTACATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.7075 |
| LEAP-Seq n confirming: | 18409 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCCAGAGACTGTGACAA |
| Suggested primer 2: | AGTGGTCACATGTGTTCGGA |