Insertion junction: LMJ.RY0402.043982_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.043982
Chromosome:chromosome 13
Location:1234150
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g570350 AKC4 (1 of 1) PTHR10566//PTHR10566:SF74 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // ABC TRANSPORTER-LIKE; conserved expressed ABC-1 like kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGGGAAACGGTTACTACCCCGCACGGA
Internal bar code:CGTCCGTCGCGTGGCGTTTCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:612
LEAP-Seq percent confirming:79.7111
LEAP-Seq n confirming:7614
LEAP-Seq n nonconfirming:1938
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTCCTCGATCTTGTTGGG
Suggested primer 2:AGCATACAAACGGTTGAGGG