Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.044002 |
Chromosome: | chromosome 12 |
Location: | 2842312 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g502350 | HEL50 | (1 of 1) K10899 - ATP-dependent DNA helicase Q1 [EC:3.6.4.12] (RECQL); DEAD/DEAH box DNA/RNA helicase, related to RECQ1 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAAGCAGGCACCGCGCCGGCAGTCCATC |
Internal bar code: | CCCCGACGCGCGGTGAGTGGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 808 |
LEAP-Seq percent confirming: | 93.4605 |
LEAP-Seq n confirming: | 7303 |
LEAP-Seq n nonconfirming: | 511 |
LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCGAGCCCCACGTATAGAG |
Suggested primer 2: | GTTGACACCGTGGTTACGTG |