| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044014 |
| Chromosome: | chromosome 17 |
| Location: | 2173665 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713025 | DNJ33 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACCTAGCCCGCCCCGCCGCCCGCCCGT |
| Internal bar code: | AGCGAGGACAAAACGGGAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 97.9316 |
| LEAP-Seq n confirming: | 7528 |
| LEAP-Seq n nonconfirming: | 159 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGGACGTGTGGATTGA |
| Suggested primer 2: | CCGCCATTTCCTGACTGTAT |