Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.044015 |
Chromosome: | chromosome 8 |
Location: | 820027 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g360801 | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTCGCAGTGCTAGCCCGCTGTAACAAC |
Internal bar code: | ACGGACCGGTTGTCCGCCTCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 934 |
LEAP-Seq percent confirming: | 98.6203 |
LEAP-Seq n confirming: | 6290 |
LEAP-Seq n nonconfirming: | 88 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACGAGAACAGGGTTGATGG |
Suggested primer 2: | CGCCTGGTACTTGTGCTGTA |