| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044016 |
| Chromosome: | chromosome 9 |
| Location: | 4805790 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397327 | (1 of 1) PTHR11139:SF68 - DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTACTGTTGCTGGAAAGCAGCACCGACA |
| Internal bar code: | TCTATATCTGTTGTTATATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 303 |
| LEAP-Seq percent confirming: | 99.2609 |
| LEAP-Seq n confirming: | 5372 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGACAGTGGTAAGGCAG |
| Suggested primer 2: | GCCTCACAAAAGCTAGGCAC |