| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044056 |
| Chromosome: | chromosome 12 |
| Location: | 1943478 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510550 | (1 of 2) PTHR12197//PTHR12197:SF145 - SET AND MYND DOMAIN CONTAINING // SET DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCGTCGTCATCGCCCCGGTCCCTCTCC |
| Internal bar code: | CCACGTTCGTTTTTGGCGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 99.1689 |
| LEAP-Seq n confirming: | 3341 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAGTTGCTCCATAACCG |
| Suggested primer 2: | GGACCCTTTGACAACTGCAT |