| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044065 |
| Chromosome: | chromosome 5 |
| Location: | 291954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233551 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCGCCAGCGGTATGTAACGCCGAAGTG |
| Internal bar code: | TAAAAAGGAGCGTAGGTGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1065 |
| LEAP-Seq percent confirming: | 99.6515 |
| LEAP-Seq n confirming: | 20590 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGATGACACTGGCTGAGG |
| Suggested primer 2: | TGCGTGTGATTTTGAGAAGC |