| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044073 |
| Chromosome: | chromosome 12 |
| Location: | 8131649 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551352 | LAO1 | Periplasmic L-amino acid oxidase, catalytic subunit; (1 of 1) 1.4.3.2 - L-amino-acid oxidase / Ophio-amino-acid oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACGTTCGTGGAGGAGCTGACAAAGCTG |
| Internal bar code: | GGGTGGGCACGGAGTTAACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 98.9323 |
| LEAP-Seq n confirming: | 7413 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGTGGGAGTGTGGGAAG |
| Suggested primer 2: | TCGAAGCAAGTACACGCAAC |