| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044088 |
| Chromosome: | chromosome 9 |
| Location: | 2893983 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388351 | PHC32 | (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 32 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTCCCTTCTGTCACTCCTTCCGCCTGC |
| Internal bar code: | CCAGGGCTCCGTCACAAATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 99.8116 |
| LEAP-Seq n confirming: | 2649 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCAGGTGTGGATGTCATA |
| Suggested primer 2: | CAGCTTCATACACACCACGG |