| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044106 |
| Chromosome: | chromosome 10 |
| Location: | 6104109 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463600 | TGL17 | (1 of 3) PTHR21493:SF87 - SN1-SPECIFIC DIACYLGLYCEROL LIPASE BETA; Putative triacylglycerol lipase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCGAGGAGGTACGGCGGATGTACGGCG |
| Internal bar code: | ATGGATGCCTGACTCGTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.2434 |
| LEAP-Seq n confirming: | 6952 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACCCTGTCCTATGTGG |
| Suggested primer 2: | ATGGGACTTACAGTGACGCC |