| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044121 |
| Chromosome: | chromosome 9 |
| Location: | 5770422 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401923 | POB6 | Proteome of basal body 6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACCCCTCCATCCCCCACCCTTACCCCTC |
| Internal bar code: | CTCTCGCCATGCGCGTCTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 91.7767 |
| LEAP-Seq n confirming: | 2154 |
| LEAP-Seq n nonconfirming: | 193 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGGAGGACAGGTTTGA |
| Suggested primer 2: | TGTACTCCCTCAGTCCCCTG |