| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.044148 |
| Chromosome: | chromosome 6 |
| Location: | 2347219 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267450 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGACTGCTCCACACAAGCACATGCACTC |
| Internal bar code: | GGGGATTGGAACGGAATGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 764 |
| LEAP-Seq percent confirming: | 98.9585 |
| LEAP-Seq n confirming: | 7031 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCATAAGGATGGGTTTG |
| Suggested primer 2: | CCATTGATCACAGCCAGTTG |