| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044160 |
| Chromosome: | chromosome 11 |
| Location: | 44656 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467527 | (1 of 1) PTHR24286:SF0 - CYTOCHROME P450 61 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTGCATGTGCTGAAACCTCGATGTTGTG |
| Internal bar code: | CACGCTAAAAGCTCCAGTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 98.1329 |
| LEAP-Seq n confirming: | 3574 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGAACCACCTGACTGTG |
| Suggested primer 2: | TGTTGACTGCATTCTCCTCG |