| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044169 |
| Chromosome: | chromosome 16 |
| Location: | 206703 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694809 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGACTGCGAGCGGGCAGGCAGGCGGTGC |
| Internal bar code: | GCGTCCTGACCCCATCGTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 2.10938 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 2506 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCCTTCAGAGCTACACG |
| Suggested primer 2: | TACTTAGTGGGCGGGATTTG |