| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044171 |
| Chromosome: | chromosome 12 |
| Location: | 5339785 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529450 | DRP5A,DRP5 | (1 of 1) PTHR11566//PTHR11566:SF44 - DYNAMIN // DYNAMIN-LIKE PROTEIN C; Dynamin-type GTPase involved in plastid division | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTGTAACAAGTACTAAAGGGCCACAGC |
| Internal bar code: | TTTCCCGACCTCGGTTTCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 53 |
| LEAP-Seq percent confirming: | 96.4602 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTGTTCTCCAGGTTGGT |
| Suggested primer 2: | CTTTGCCCTCTGTGCCTTAG |