Insertion junction: LMJ.RY0402.044230_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.044230
Chromosome:chromosome 6
Location:6048414
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g289450 (1 of 1) IPR001194//IPR005112 - DENN domain // dDENN domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGATGCATGGTGCATTGCGGGCACCAAC
Internal bar code:TCCGATTGGTGACTACTCGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:495
LEAP-Seq percent confirming:96.9473
LEAP-Seq n confirming:8543
LEAP-Seq n nonconfirming:269
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCACCACAAGCATATTCG
Suggested primer 2:CATGACACACCTTCCACCTG