| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044326 |
| Chromosome: | chromosome 12 |
| Location: | 1150710 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490150 | HEL48 | (1 of 1) K03654 - ATP-dependent DNA helicase RecQ (recQ); DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCACGGCGCCGGCGCCGATCGCAGCGA |
| Internal bar code: | ATTGCCTGCCGCTAGTCCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 99.7306 |
| LEAP-Seq n confirming: | 2221 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAAAGGAACATGCGGTG |
| Suggested primer 2: | CATACACGTCCCGCTAACCT |