| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044356 |
| Chromosome: | scaffold 34 |
| Location: | 16303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre34.g759147 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGCCTGGCCTGAACAGCTGCAGTGCAG |
| Internal bar code: | TATGAAAAATATTGAGCACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 564 |
| LEAP-Seq percent confirming: | 77.1667 |
| LEAP-Seq n confirming: | 463 |
| LEAP-Seq n nonconfirming: | 137 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCTGTGGTGCTTTACGGG |
| Suggested primer 2: | GTAGTTGTCCCAGGCGATGT |