| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044369 |
| Chromosome: | chromosome 2 |
| Location: | 4047089 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097227 | Found in Chlamydomonas and Volvox but not strongly conserved elsewhere | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGGTATGTGTGTGTATAGGGGGTAGCTG |
| Internal bar code: | GTGTTTATACGGGAACCGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 99.6721 |
| LEAP-Seq n confirming: | 608 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGAGGGATGGGATGGGT |
| Suggested primer 2: | TCGTGTGTGTGTCCAATCCT |