| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044375 |
| Chromosome: | chromosome 2 |
| Location: | 6092068 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113100 | (1 of 1) PTHR14154:SF3 - MITOCHONDRIAL PYRUVATE CARRIER 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGCTTGGTGTTTGGTTTGCCTAAGGCA |
| Internal bar code: | CTCGAAAGTGTGGGCCAGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 89.5963 |
| LEAP-Seq n confirming: | 2308 |
| LEAP-Seq n nonconfirming: | 268 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTACGGCCACATGACAC |
| Suggested primer 2: | AGGACGTACCAGGACAATGC |