| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044413 |
| Chromosome: | chromosome_9 |
| Location: | 3667873 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390615 | FAP12 | Triacylglycerol lipase and Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTGATACATAACAGCTGTGCTGCGTTCAT |
| Internal bar code: | TAGGGATACCATGCGGCGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1003 |
| LEAP-Seq percent confirming: | 98.7776 |
| LEAP-Seq n confirming: | 3798 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCAGCTAATGCCTTGCGT |
| Suggested primer 2: | GAAGAAAGGGAGCAGACGTG |