| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.044434 |
| Chromosome: | chromosome 12 |
| Location: | 4445981 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521350 | SLY1 | (1 of 1) PTHR11679:SF2 - SEC1 FAMILY DOMAIN-CONTAINING PROTEIN 1; SM/Sec1-family protein, SLY1 homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCATTCCCTGCTTACAAGCAAGGCGCGT |
| Internal bar code: | GCGGTGACTGAAAGATGAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 97.3546 |
| LEAP-Seq n confirming: | 9826 |
| LEAP-Seq n nonconfirming: | 267 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGCAAGACTACCCCAT |
| Suggested primer 2: | TGCGGGTAAGGGTTAACTTG |