| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044450 |
| Chromosome: | scaffold 24 |
| Location: | 119141 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755997 | PHC18,FAP150 | Pherophorin-like Flagellar Associated Protein 150; (1 of 71) PF12499 - Pherophorin (DUF3707) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAACAGGCTTGGAAGCCGGGGTCAGAG |
| Internal bar code: | ACTGTAGGCGAACGCTACGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 99.8036 |
| LEAP-Seq n confirming: | 20834 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACAACATTATGACCCG |
| Suggested primer 2: | CATCCTTGAAGGGCTGGTAA |