| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044453 |
| Chromosome: | chromosome 9 |
| Location: | 770364 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402150 | (1 of 1) IPR002734//IPR024072 - Bacterial bifunctional deaminase-reductase, C-terminal // Dihydrofolate reductase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTCCGCAAAGCCGCCACCACCTCCACG |
| Internal bar code: | GCGCGCCGAAAAAACCAGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 66 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGGTCACAGACCACACG |
| Suggested primer 2: | GTACGCAAAAGCATGAGCAA |