| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.044472 |
| Chromosome: | scaffold 18 |
| Location: | 118602 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748997 | (1 of 3) PTHR11132//PTHR11132:SF64 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCAACGACCAGGCTGAATGCCTTCACT |
| Internal bar code: | GTCGTCAAGAAGACGGTGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 876 |
| LEAP-Seq percent confirming: | 99.1536 |
| LEAP-Seq n confirming: | 820 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATCCGCCCTCACCTCTCT |
| Suggested primer 2: | AAGGCAATGTGTCCCTTACG |