| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044474 |
| Chromosome: | chromosome 10 |
| Location: | 1485752 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428734 | (1 of 2) IPR000104//IPR000945 - Antifreeze protein, type I // Dopamine beta-hydroxylase-related | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGCAAAACACGCATACAAAATACACAT |
| Internal bar code: | GTGGGGCATCGCCAGACGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 72.5806 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAAACCTGCCTGAGAAGT |
| Suggested primer 2: | TTGTTCCCTTCCACAACACA |