Insertion junction: LMJ.RY0402.044485_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.044485
Chromosome:chromosome 13
Location:3741924
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTCGTGAGGAATTCGACCTGGCCGTGTC
Internal bar code:CTGTTTAGGCCCAAGCATTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:416
LEAP-Seq percent confirming:97.8313
LEAP-Seq n confirming:406
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAACGAAATTGTGAGCCC
Suggested primer 2:GAGTGGCGCTACAGTGACAA