| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044523 |
| Chromosome: | chromosome 9 |
| Location: | 3637413 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390319 | (1 of 1) PTHR10663:SF42 - PROTEIN BOI2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTTAGTGTAAACTAAAAGGGGTAGCAA |
| Internal bar code: | CGGGCTCGTGGCGCGAGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 757 |
| LEAP-Seq percent confirming: | 99.8768 |
| LEAP-Seq n confirming: | 811 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTTTGGTGACCTTGCTG |
| Suggested primer 2: | TGTGGAGTTTGCCTCCTTCT |