| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044534 |
| Chromosome: | chromosome_3 |
| Location: | 9154682 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g212305 | FAP181 | Flagellar Associated Coiled-Coil Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCAGGATGGGCAGCGACAGGGCAGCGTGTG |
| Internal bar code: | GGGGGACCTGGGTCGGGTAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 762 |
| LEAP-Seq percent confirming: | 95.3179 |
| LEAP-Seq n confirming: | 1649 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCTCTCTTCACACGACC |
| Suggested primer 2: | CCACACTCTCATGCACCTTG |